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Synergy_H4_Applications

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Examples of SynergyH4Applications:
AlphaScreen/AlphaLISAApoptosisBacterial adhesionBRET/BRET2Cell adhesionCell expressionCell proliferationCell viability &CytotoxicityChemotaxisColorimetric assaysCytokine analysisDNA quantificationeGFP quantificationELISAEnzyme activityEnzyme kineticsFRETHeterogeneous TRF (DELFIA)Homogeneous TRF (LANCE)
ImmunoassaysKinase activityMetabolic activityMitochondrial membrane potentialNADH/NADPHNa+/H+exchangeNucleic acid quantificationOxidative burstOxidation reactionsPCR product quantification & qualityProtease activityProtein quantificationReceptor binding studiesReporter geneSignal TransductionSingle Nucleotide PolymorphismsAbs/FI spectral scanningToxicology
Examples ofAbsorbance/Photometric Applications:
Assay TargetAlamarBlue®Cell Proliferation, Viability & CountingAmplex®Red Enzyme ActivityphosphoELISA KinasesVybrant assay C12 Rezarurin Metabolic LabelingVybrant MTT Cell Proliferation Assay Kit Cell CountingLipid Peroxidation Assay Kit (using MDA) Oxidative StressNeutral Red Uptake Assay Cell ProliferationCellTiter 96®Cell Proliferation Assay (MTT) Cell ProliferationDC Protein, Bradford, Lowry & BCA Protein Assays Protein QuantificationOxiSelect™Superoxide Dismutase Activity Assay Oxidative StressPierce 660nm Protein Assay Protein QuantificationGlycoprotein Carbohydrate Estimation Kit Glycoprotein QuantificationColorimetric Protease Assay Kit ProteaseMicro BCA Protein Assay Kit Protein QuantificationFluoReporter Biotin-XX Protein Labelling Kit BiotinSulforhodamine B Assay Cell ProliferationWST-1 Cell Proliferation Assay Cell CountingCell Counting Kit-8 (CCK-8) Cell CountingIDEXX FlockChek™Avian Influenza Multi-species ELISA Assay Avian InfluenzaMelamine ELISA Assay Food SafetyCaspACE™Assay ApoptosisMTT Assay Cell ProliferationGrowth Assay (OD at 600nm) Cell GrowthHaemoglobin denaturation Assay Haemoglobin
Examples ofFluorescence Intensity Applications:
Assay TargetAdapta®Universal Kinase Assay KinasesAlamarBlue®Cell Proliferation, Viability & CountingAmplex®Red Enzyme ActivityCyQuant®Cell Proliferation Assay Kit Cell ProliferationEnzCheck ProteasesFluo-4 AM GPCRGeneBLAzer GPCR GPCRLIVE/DEAD®Viability/Cytotoxicity Kit ApoptosisNDP Sensor Assay KinasesOmnia®Kinase Assays KinasesPiPer™Phosphate Assay Kit PhosphatasesSYTOX®stains ViabilityVivid®CYP450 Cytochrome P450Z'-LYTE™KinasesQuant-iT™PicoGreen®dsDNA Assay Kit Nucleic Acid QuantificationTranscreener® GDP FI Assay GTPaseFura-2 GPCRIndo-1 GPCRFluxOR™Potassium Ion Channel Assay Kits Ion ChannelsOxiSelect™ROS Assay Kit Oxidative StressCellTiter-Fluor™Cell Viability Assay Cell ViabilityBD™Ratiometric Calcium Assay Kit GPCRFluoroBlok Cell Invasion Assays (using calcein AM) Cell Migration
Examples ofLuminescence Applications:
Assay TargetCellTiter-Glo®Luminescent Cell Cell ProliferationDual-Glo®Luciferase Assay Gene ExpressionDual-Luciferase®Reporter (DLR™) AssayGene ExpressionBright-Glo™ Luciferase Assay Gene ExpressionSteady-Glo®Luciferase Assay Gene ExpressionONE-Glo™ Luciferase Assay Gene ExpressionP450-Glo™ Assay CYP ActivitiesADP-Glo™ Assay KinaseCaspase-Glo®3/7 Assay ApoptosiscAMP-Glo™ Assay GPCRGloSensor™ cAMP Assay GPCRAequorin GPCRPhotina®GPCRBritelite™ plus Reporter Gene Assay Gene ExpressionSteadylite plus™ Reporter Gene Assay Gene ExpressionNeolite Reporter Gene Assay Gene ExpressionATPlite™ Luminescence Assay Cell Proliferation/KinasesaCella -TOX™ Bioluminescence Cytotoxicity Assay Cell DeathToxiLight® Non-destructive Cytotoxicity BioAssay Kit Cell Death/Cell ProliferationATP quantification assay (luciferine-luciferase) ATP Quantification
Examples ofTime-Resolved Fluorescence Applications:
Assay TargetLanthaScreen™Kinase Assay KinasesLanthaScreen™Cofactor Recruitment Assays Nuclear ReceptorsLanthaScreen™Cellular assay KinasesTranscreener®ADP TR-FRET Assay KinasesLANCE®cAMP Detection Kit GPCRDELFIA®cAMP Kit GPCRDELFIA®Tyrosine Kinase Kit KinasesDELFIA®EuTDA Cell Cytotoxicity Kit CytotoxicityDELFIA®Cell Proliferation Kit Cell ProliferationPI3-Kinase HTRF Assay Cell ProliferationHTRF®HTplex™cAMP and IP-One Assay GPCRHTRF®cAMP femto 2 Kit GPCRHTRF®KinEASE™ Kits KinasesHTRF®heparanase Assay HeparanasesHTRF®Ubiquitin Assays UbiquitinasesHuman Insulin TRF-Pincer™Assay InsulinTag-lite®Receptor Ligand Binding Assay GPCRCellul'erk GPCRHTScan®Kinase Assays Kinases
Examples ofFluorescence Polarization Applications:
Assay TargetPolarScreen™KinasesPolarScreen™Competitive Binding Assays Nuclear ReceptorsTranscreener®AMP/GMP Assay PDE’sTranscreener®GDP FP Assay GTPaseTranscreener®UDP Assay UDP-glycosyltransferasesTranscreener®ADP2 FP Assay Kinases[FP]2 cAMP
Examples ofAlphaScreen Applications:
Assay TargetAlphaScreen®cAMP Assay GPCRAlphaScreen®SureFire®Assay GPCRAlphaLISA®Immunoassay Kits Biological Markers (see below)Biologics: IgA, IgG, IgM, CHO-P, MSO-P, E.Coli HCP, Residual Protein AAngiogenesis: TNFα, VEGF, VEGFB, VEGFC, VEGFDCancer: AFP, CA125, CXCL11/I-TAC, EGF-R, EPO, ERBB2/HER2, IFNβ, MMP1, MMP2, MMP9,β-NGF, PSACardiovascular: Myeloperoxidase, NT-proBNP, PCSK9, Plasminogen, Renin/Prorenin, tPA, ICAM-1Neurodegeneration: Aβ1-40, Aβ1-42,sAPPα, sAPPβ, Aβ1-15/16Metabolic: Adiponectin, GH, GLP-1, IGF1, IGF2, Insulin, Leptin, Prolactin, AlbuminVirology: HIV p24Inflammation: CCL2/MCP1, CCL3/MIP-1α, CCL4/MIP-1β, COMP, CRP, CXCL10/IP-10, G-CSF, IFNα, IFNγ, IL1α, IL1β, IL2, IL3, IL4, IL5, IL6, IL7, IL8, IL10, IL12 (p70), IL13, IL17, IL18, CXCL1/GROα, CXCL9/MIG, IL15
Enabling technologies covering the full spectrum of targets:

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Synergy_H4_Applications