BioFire (FilmArray) Multiplex PCR Assays - Pathology

BioFire(FilmArray) Multiplex PCR Assays
TeresaFinken, MT(ASCP)
Panels Available
Respiratory Viral PanelBlood Culture Identification PanelGastrointestinal Panel
Methodology
Multiplex Real-Time PCR AssayThese panels test for multiple targets simultaneouslyThis methodology is real-time PCR meaning that it amplifies and detects at the same time
What does this Mean to You?
One sampleOne testMultiple results1.5-hr turnaround time
Respiratory Viral Panel Targets
VirusesAdenovirusCoronavirusHKU1CoronavirusNL63Coronavirus229ECoronavirusOC43HumanMetapneumovirusHuman Rhinovirus/EnterovirusInfluenzaAInfluenzaA/H1InfluenzaA/H1-2009Influenza A/H3tapneumovirus
InfluenzaBParainfluenza1Parainfluenza2Parainfluenza3Parainfluenza4Respiratory Syncytial VirusBacteriaBordetellapertussisChlamydophilapneumoniaeMycoplasmapneumoniae
Blood Culture Id Panel Targets
Gram+ BacteriaEnterococcusListeriamonocytogenesStaphylococcusStaphylococcusaureusStreptococcusStreptococcusagalactiaeStreptococcuspyogenesStreptococcuspneumoniaeYeastCandidaalbicansCandidaglabrataCandidakruseiCandidaparapsilosisCandidatropicalisAntibioticResistancemecA- methicillin resistantvanA/B -vancomycinresistantKPC -carbapenemresistant
Gram– BacteriaAcinetobacterbaumanniiHaemophilusinfluenzaeNeisseriameningitidisPseudomonasaeruginosaEnterobacteriaceaeEnterobactercloacae complexEscherichia coliKlebsiellaoxytocaKlebsiellapneumoniaeProteusSerratiamarcescens
Gastrointestinal Panel Targets
BacteriaCampylobacter(jejuni, coliandupsaliensis)Clostridiumdifficile(toxin A/B)PlesiomonasshigelloidesSalmonellaYersiniaenterocoliticaVibrio (parahaemolyticus,vulnificusandcholerae)VibriocholeraeDiarrheagenicE. coli/ShigellaEnteroaggregativeE. coli (EAEC)EnteropathogenicE. coli (EPEC)EnterotoxigenicE. coli (ETEC)lt/stShiga-like toxin-producing E. coli (STEC) stx1/stx2E. coli O157Shigella/EnteroinvasiveE. coli (EIEC)
VirusesAdenovirusF 40/41AstrovirusNorovirus GI/GIIRotavirus ASapovirus(I, II, IV and V)ParasitesCryptosporidiumCyclosporacayetanensisEntamoebahistolyticaGiardialamblia
Sample Requirements
Respiratory Viral PanelNasal WashNasal SwabSputumRespiratory specimen in Viral Transport MediumGastrointestinal PanelRaw stool to be transferred to ETMStool in ETM
Blood Culture ID PanelBlood culture bottle that has been flagged positive by theBactiAlert orVersatrekAnalyzers
Positive Results SinceImplementaion
These are just a few…Limited exposure to potentially hazardous bacterial isolates in blood culturesFaster treatment and more guided treatment of patients at the point of entry and after admissionQuick diagnosisFaster antimicrobial therapy implementation to speed recovery and reduce morbidityAdmitting a patient versus not admitting a patient
Questions…